ABSTRACT Biomass is a valuable indicator of biological production in any ecosystem and represents a proxy of secondary production in the case of zooplankton. This short communication aims to report zooplankton biomass values in the waters of the Campeche Canyon, southern Gulf of Mexico, during the “Nortes” storm season of 2011 and to explore their relationship with the hydrography and the circulation pattern. The results showed the presence of cyclonic and anticyclonic eddies associated with high and low water density at the base of the pycnocline. The highest values of zooplankton biomass (>40 g 100 m-3) were observed in regions that presented higher water density values (~26.1 kg m-3). In comparison, the lowest zooplankton biomass values (<5 g 100 m-3) were associated with low water densities (<25.6 kg m-3). The results presented here contribute to elucidating the role that physical forcing plays on the zooplankton biomass of the region, particularly during a season in which the passage of extreme storms is widespread.

Abstract Background Satellite cells are tissue-specific stem cells primarily responsible for the regenerative capacity of skeletal muscle. Satellite cell function and maintenance are regulated by extrinsic and intrinsic mechanisms, including the ubiquitin–proteasome system, which is key for maintaining protein homeostasis. In this context, it has been shown that ubiquitin-ligase NEDD4-1 targets the transcription factor PAX7 for proteasome-dependent degradation, promoting muscle differentiation in vitro. Nonetheless, whether NEDD4-1 is required for satellite cell function in regenerating muscle remains to be determined. Results Using conditional gene ablation, we show that NEDD4-1 loss, specifically in the satellite cell population, impairs muscle regeneration resulting in a significant reduction of whole-muscle size. At the cellular level, NEDD4-1-null muscle progenitors exhibit a significant decrease in the ability to proliferate and differentiate, contributing to the formation of myofibers with reduced diameter. Conclusions These results indicate that NEDD4-1 expression is critical for proper muscle regeneration in vivo and suggest that it may control satellite cell function at multiple levels.

Abstract Chelates are nutrient-rich compounds that enhance the condition of plant tissues as micronutrients. Micronutrient deficiencies particularly iron (Fe) and zinc (Zn) leads to various problems for plant including chlorosis and necrosis etc. An adequate intake of Fe and Zn etc. is required by the human body. Biofortification of cereals with Fe and Zn is seen as a cost-effective solution to the problem of Fe and Zn deficiencies as well. In recent decades, many chelating compounds have been established and incorporated into agricultural systems. The most recent formulation involves the use of amino acids synthesized with one or more nutrient ions to improve fertilizer efficiency and better respond to environmental conservation. In addition to its primary function as a source of micronutrients, aminochelled are an active nitrogen (N) stimulant in plant nutrition, preventing the negative effects of basic N fertilizers like urea. The use of amino chelates, rather than just chemical fertilizers, has been shown to provide better production and quality as well as higher nutritional concentrations in several experiments. Furthermore, this review sheds light on various aspects of amino chelates fertilizers including types, history, and their effects on agricultural crops. In spite of amino chelates fast dominance in many countries’ fertilizer countries, there is not enough scientific data and knowledge on the specific reactions of plants to biotic and abiotic stresses from amino fertilizers.

Resumo Os quelatos são compostos ricos em nutrientes que melhoram a condição dos tecidos vegetais como micronutrientes. Deficiências de micronutrientes, particularmente ferro (Fe) e zinco (Zn), levam a vários problemas para as plantas, incluindo clorose e necrose, etc. A ingestão de uma quantidade adequada de Fe e Zn, etc., é exigida pelo corpo humano. A biofortificação de cereais com Fe e Zn também é vista como uma solução econômica para o problema das deficiências de Fe e Zn. Nas últimas décadas, muitos compostos quelantes foram estabelecidos e incorporados em sistemas agrícolas. A formulação mais recente envolve o uso de aminoácidos sintetizados com um ou mais íons nutrientes para melhorar a eficiência do fertilizante e responder melhor à conservação ambiental. Além de sua função primária como fonte de micronutrientes, os aminoquelados são um estimulante de nitrogênio (N) ativo na nutrição das plantas, evitando os efeitos negativos de fertilizantes nitrogenados básicos como a ureia. O uso de aminoquelatos, ao invés de apenas fertilizantes químicos, tem mostrado proporcionar melhor produção e qualidade, bem como maiores concentrações nutricionais em vários experimentos. Além disso, a presente revisão lança luz sobre vários aspectos dos fertilizantes aminoquelatos, incluindo tipos, história e seus efeitos nas culturas agrícolas. Apesar do domínio rápido dos aminoquelatos em muitos países de fertilizantes, não há dados científicos suficientes e conhecimento sobre as reações específicas das plantas aos estresses bióticos e abióticos dos fertilizantes amino.

ABSTRACT Motivation is a fundamental element for the practice of physical activity and the feeling of satisfaction with life. However, little is known about the role of such psychological variables in the lifestyle of Chilean university students. This study aimed to analyse the relationship between life satisfaction and the regulations of motivation and to compare such variables by physical activity level and sedentary behaviour of university students. The sample comprised 95 Chilean university students (63.2% female), with a mean age of 20.92 ± 1.98 years. The instruments were the IPAQ, the Satisfaction with Life Scale, and the Behavioural Regulation in Exercise Questionnaire. Descriptive measures, Pearson’s correlation coefficient, and Student’s t-tests were used to analyse the data. The results revealed positive and significant correlations between life satisfaction and intrinsic (r = 0,44), integrated (r = 0,38), and identified (r = 0,41) regulation. Students with high levels of physical activity scored higher for intrinsic, integrated, and identified regulation. Those who reported low sedentary behaviour showed higher identified regulation. Therefore, it is suggested that students’ motivational regulation may differ on some dimensions as those with higher levels of physical activity show more adaptive motivational regulation. Identified regulation also seems to play an important role in decreasing sedentary behaviour.

RESUMO A motivação é elemento fundamental para a prática de atividade física e o sentimento de satisfação com a vida. Contudo, pouco se sabe sobre o papel de tais variáveis psicológicas no estilo de vida de estudantes universitários chilenos. Este estudo teve como objetivos analisar as relações entre a satisfação com a vida e as regulações da motivação, e comparar tais variáveis em função do nível de atividade física e do comportamento sedentário de estudantes universitários. A amostra foi composta por 95 estudantes universitários chilenos (63,2% do gênero feminino), com idade média de 20,92 ± 1,98 anos. Os instrumentos avaliados foram o IPAQ, a Escala de Satisfação com a Vida e o Questionário de Regulação do Comportamento do Exercício. Medidas descritivas, coeficientes de correlação de Pearson e testes t de Student foram utilizados para analisar os dados. Os resultados revelaram correlações positivas e significativas entre satisfação com a vida e regulação intrínseca (r = 0,44), integrada (r = 0,38) e identificada (r = 0,41). Os estudantes com alto nível de atividade física obtiveram maiores pontuações para regulação intrínseca, integrada e identificada. Os indivíduos que reportaram menor comportamento sedentário possuíam maior regulação identificada. Desse modo, sugere-se que a regulação motivacional dos estudantes pode ser diferente em algumas dimensões, sendo que os indivíduos com maior nível de atividade física semanal demonstram regulações motivacionais mais adaptativas. A regulação identificada também parece desempenhar um papel importante para a redução do comportamento sedentário.

ABSTRACT The animal feed industry is continuously researching new feed additives to substitute other materials, reduce costs, or add value to the final product. The microalgae Arthrospira maxima, cultivated using wastewater as a nutritional source, was evaluated as a feed additive by including 2, 4, and 6% in isocaloric and isoprotein diets for laying hens. Five replicates per treatment with 5 hens per cage were used during an experimental period of 28 days. Productive behavior and egg characteristics (quality, fatty acid profile, cholesterol level) were evaluated. The inclusion of microalgae up to 4% in diets for 52-week-old laying hens did not affect productive performance (egg production, egg weight, egg mass, FCR, shell thickness, and Haugh units). No effects were observed on the cholesterol level or the concentration of fatty acids in the eggs, but more information is needed to determine if the microalgae drying or storage process can generate variations of these results. The inclusion of 6% microalgae produced an acceptable egg yolk color for the local market. The results indicate that this material can be used as a protein source up to an inclusion of 4% in the diet, and hens may need an adaptation period to maintain production at the 6% inclusion level.

Abstract The Western Central Valley (WCV) of Costa Rica is an area of interest, due to its high concentration of population and economic activity, presenting itself as a tropical monsoontype atmospheric basin (AB), with well-defined climatic seasons (dry and rainy). The present study proposes the assessment of low carbon steel (CS) atmospheric corrosion, based on ISO 9223 (2012) and associated standards. A general analysis of the atmospheric basin effect was initially performed on these data, followed by the basic modeling of air pollutants and meteorological parameters. The WCV is an area of low contamination, which corresponds to a C2 or C3 category, according to ISO 9223. It mainly shows significant climatic seasons (dry and rainy) effects on the initial corrosion rates, but obtaining similar annual corrosion results for them. The ISO 9223 annual atmospheric corrosion model overestimated the actual obtained corrosion values, whereas linear or logarithmic models gave better results, especially when time and/or time of wetness (TOW) were considered as variables.

Resumen Introducción: Hasta donde se tiene conocimiento, la investigación que se presenta constituye el primer trabajo multicéntrico en México que estudia el desarrollo de la aptitud clínica en unidades formadoras de cardiólogos. Objetivo: Determinar el grado de desarrollo de la aptitud clínica en residentes de cardiología en tres unidades médicas de alta especialidad. Métodos: Diseño transversal multicéntrico. Se analizaron todos los estudiantes del ciclo académico 2019-2020. Se construyó un instrumento que evaluó la aptitud clínica a partir de ocho indicadores y 170 ítems; la validez conceptual/de contenido y la confiabilidad fueron valoradas por cinco cardiólogos con experiencia docente y en investigación educativa. Resultados: Por indicador y año de residencia se observaron diferencias estadísticas significativas en la sede CMN20Nov; en HCSXXI e INCICh se observaron diferencias estadísticamente significativas en uno de ocho indicadores. Se estimaron diferencias entre residentes R1 (n = 41) de las tres sedes por indicador, con significación estadística en tres de ocho indicadores. El resultado fue semejante al comparar R2 (n = 35) y R3 (n = 43). Conclusiones: El grado de desarrollo de la aptitud clínica se puede considerar medio en las tres sedes académicas, probablemente debido a que el instrumento exploró situaciones clínicas problematizadas que exigieron del residente la reflexión crítica de su experiencia clínica.

Abstract Introduction: To the best of our knowledge, the research herein presented is the first multicenter study in Mexico to analyze the development of clinical aptitude in medical units that train cardiologists. Objective: To determine the degree of development of clinical aptitude in cardiology residents at three High Specialty Medical Units. Methods: Multicenter, cross-sectional design. All students of the 2019-2020 academic year were included in the study. An instrument was constructed that evaluated clinical aptitude based on eight indicators and 170 items; conceptual/content validity and reliability were assessed by five cardiologists with teaching and educational research experience. Results: By indicator and year of residence, significant statistical differences were observed in the CMN20Nov academic site. At HCSXXI and INCICh, statistically significant differences were observed in one of eight indicators. Differences between R1 residents (n = 41) of all three academic sites were estimated by indicator, with statistical significance being recorded in three of eight indicators. Between R2 (n = 35) and between R3 residents (n = 43), the result was similar. Conclusions: The degree of clinical aptitude development can be considered intermediate in all three academic sites, probably because the instrument explored problematized clinical situations that required the residents to critically reflect on their clinical experience.

RESUMEN Se presenta la evolución histórica de la certificación de especialistas en medicina familiar en México, a 35 años de su fundación. Asimismo, se abordan brevemente los procesos de evaluación de los sustentantes y la importancia de mantener la «vigencia de la certificación». Se realiza el benchmarking de la certificación en otros países con procesos de certificación bien definidos y consolidados. Al final, se presentan los retos más inmediatos de la certificación en México para esta especialidad.

ABSTRACT: The historical evolution of the Certification of Specialists in Family Medicine in Mexico is presented, 35 years after its foundation. Likewise, the evaluation processes of the applicants and the importance of maintaining the “Certification Validity” are briefly addressed. Certification benchmarking is carried out in other countries with well-defined and consolidated certification processes. At the end, the most immediate challenges of the Certification in Mexico for this specialty are presented.

Abstract The objective of this study was to produce organic fodder based on corn and Yorimón bean as an alternative to conventional fodders in the Lagunera Region. The experiment was conducted in the spring-summer cycle from April to August 2017. In each useful plot, three corn plants and three Yorimón bean plants were harvested, which were finely chopped and mixed to form a balanced compound of which one kilogram was used for bromatological analyses. A randomized block design with four repetitions was used. In the CP of organic and inorganic fodders, no significant differences were observed (p> 0.05) and the values found were within the optimal values for cows in production. CP correlated positively with TDN and NEL (p< 0.01). The quality of the fodder combined between corn and Yorimón bean showed that it is an alternative to conventional fodders.

Resumen El objetivo de este estudio fue producir forraje orgánico a base de maíz y frijol Yorimón como alternativa a los forrajes convencionales en la Región Lagunera. El experimento se realizó en el ciclo de primavera-verano de abril a agosto de 2017. En cada parcela útil se cosecharon tres plantas de maíz y tres de frijol, las cuales fueron finamente picadas y mezcladas para formar un compuesto balanceado del cual se utilizó un kilo para los análisis bromatológicos. Se utilizó un diseño de bloques al azar con cuatro repeticiones. En PC de los forrajes orgánicos e inorgánicos, no se observaron diferencias significativas (p> 0.05) y los valores encontrados estuvieron dentro de los valores óptimos para vacas en producción. PC correlacionó positivamente con la TND y ENL (p< 0.01). La calidad del forraje combinado entre maíz y frijol Yorimón mostró que es una alternativa a los forrajes convencionales.

Abstract: To identify success factors and weaknesses in coverage in the main insurance variables and regional distribution in México, data on protected animal units, the amount of premiums contributed by producers, the federal and state governments and the sum insured were used. With resources paid in 83% from subsidies and 17% from farmers, and with the participation of five insurance institutions and an insurance fund, up to 83 million animal units were protected, covering all producers in the National Livestock Register. With cluster analysis, three groups of states were obtained and with the analysis of variance, significant statistical differences were found (p < 0.05) in the amount of premiums paid, average protected value and number of emergency and disaster declarations. The flexibility of the program to incorporate insurance schemes and the subsidy of the premium with public resources were identified as success factors.

Resumen: Con el objetivo de identificar factores de éxito y debilidades en la cobertura de seguros catastróficos y las características de su distribución regional en México, se trabajaron datos de unidades animal protegidas, primas aportadas por los productores, gobierno federal y gobiernos estatales y suma asegurada. Se encontró que con recursos aportados en un 83% de subsidio y 17% de los productores, cinco instituciones de seguros y un fondo de aseguramiento protegieron hasta 83 millones de Unidades Animal de la totalidad de los productores inscritos en el Padrón Ganadero Nacional. Con análisis de conglomerados se obtuvieron tres grupos de entidades federativas y con el análisis de varianza se encontraron diferencias estadísticas significativas (p < 0.05) en monto de primas, valor protegido y número de declaratorias de emergencia y desastre. Se identificaron como factores de éxito la flexibilidad del programa para incorporar esquemas de seguros y el subsidio de la prima con recursos públicos.

Resumen Introducción. La creciente resistencia bacteriana a los antibióticos representa una amenaza mundial de salud pública. Las excreciones y secreciones larvarias derivadas de moscas necrófagas de la familia Calliphoridae podrían configurar una fuente promisoria para contrarrestar sus efectos. Objetivo. Comparar la actividad antimicrobiana de las excreciones y secreciones larvarias nativas, y de las mayores y menores de 10 kDa de Calliphora vicina y Sarconesiopsis magellanica (Diptera: Calliphoridae). Materiales y métodos. El bioensayo se hizo a partir de la técnica de turbidimetría y en el caso de las excreciones y secreciones menores de 10 kDa se determinó la concentración inhibitoria mínima (CIM). Resultados. Las excreciones y secreciones nativas y las menores de 10 kDa de C. vicina y S. magellanica, evidenciaron una potente actividad antibacteriana contra tres cepas de Staphylococcus aureus y cuatro bacterias Gram negativas, siendo las menores de 10 kDa más efectivas que las nativas en las dos especies de moscas evaluadas. Además, las menores de 10 kDa presentaron la misma efectividad, aunque en las pruebas de CIM se observó que las de S. magellanica fueron más potentes en todas las bacterias evaluadas, excepto contra la cepa de S. aureus ATCC 25923. Las mayores de 10 kDa no inhibieron el crecimiento bacteriano. Conclusión. Los resultados validaron, en general, que estas sustancias son fuente importante para el aislamiento y la caracterización de agentes antimicrobianos.

Abstract Introduction: The growing resistance to antibiotics worldwide represents a global threat to public health. The larval excretions and secretions derived from necrophagous flies from the Calliphoridae family could represent a promising source for counteracting their effects. Objective: To compare the antimicrobial activity of Calliphora vicina and Sarconesiopsis magellanica (Diptera: Calliphoridae) native excretions and secretions and those weighing more than 10 kDa and less. Materials and methods: We used the turbidimetry technique for the bioassay; we determined the minimum inhibitory concentration (MIC) for excretions and secretions weighing less than 10 kDa. Results: Calliphora vicina and S. magellanica native excretions and secretions and those weighing less than 10 kDa exhibited potent antibacterial activity against three Staphylococcus aureus strains and four Gram-negative bacteria; those weighing less than 10 kDa were more effective than the native ones in the two species of flies evaluated here. Furthermore, excretions and secretions weighing less than 10 kDa had the same effectiveness, except in the MIC trials where S. magellanica excretions and secretions weighing less than 10 kDa were more potent against all the bacteria evaluated, except for S. aureus ATCC 25923. Excretions and secretions weighing more than 10 kDa did not inhibit bacterial growth. Conclusions: These results potentially validate these substances as an important source for isolating and characterizing antimicrobial agents.

Resumen Florentín, J. E.; E. L. Cabral & R. M. Salas. 2022. Lectotipificaciones de nombres ligados a Galianthe (Rubiaceae) para la flora argentina. Darwiniana, nueva serie 10(1): 29-34. Durante la preparación del tratamiento taxonómico del género Galianthe para la Flora Argentina, se hallaron varios nombres cuya tipificación debió ser resuelta. Por este motivo, 11 lectotipos son designados, con el propósito de contribuir con el esclarecimiento nomenclatural de estos nombres. Por otro lado, se presentan comentarios acerca de la distribución y hábitat de las especies de Galianthe en la Argentina.

Abstract Florentín, J. E.; E. L. Cabral & R. M. Salas. 2022. Lectotypification of names linked to Galianthe (Rubiaceae) for the Argentine flora. Darwiniana, nueva serie 10(1): 29-34. During the preparation of the taxonomic treatment of the genus Galianthe for the Flora of Argentina, several names were found in need of typification. For this reason, 11 lectotypes are designated here, hence contributing to the nomenclatural clarification of these names. Additionally, we provide comments about the distribution and habitat of Galianthe species in Argentina.

Abstract Soil is the base of any ecosystem since it conserves nutrients and water for plant roots including agriculture and plantations. In dry and semi-arid places across the world, including the UAE, sandy soils are common. Their fertility is extremely low, and production is hampered by a number of agronomic challenges. Soil conditioner sources like bentonite and chicken manure might be used to improve the poor sandy soil attributes and hence boost soil productivity. From November 2019 to March 2020, an experiment was conducted to investigate the growth rates of Bougainvillea following bentonite and chicken manure amendments to sandy soil taken from Lehbab, Dubai. Bougainvillea was evaluated for its plant height (cm), max length of primary branch (cm), the number of leaves per plant, number of secondary branches, shoot weight (g), root length (cm), root weight (g), root/shoot ratio, chlorophyll contents, and chlorophyll a* and b*. In this experiment, a complete randomized design (CRD) with five treatments was used (10 replications per treatment). According to the findings, bentonite and chicken manure additions considerably influence the productive properties of sandy soil, as indicated by Bougainvillea growth. Additionally, the research suggests that Bougainvillea may be efficiently planted with 10% bentonite and 15% chicken manure applied to sandy soil, resulting in the healthiest plants compared to other amendments.

Resumo O solo é a base de qualquer ecossistema, incluindo agricultura e plantações, pois conserva nutrientes e água para as raízes das plantas. Em lugares secos e semiáridos em todo o mundo, incluindo os Emirados Árabes Unidos, os solos arenosos são comuns. Sua fertilidade é extremamente baixa e a produção é prejudicada por uma série de desafios agronômicos. Fontes de condicionador de solo, como bentonita e esterco de galinha, podem ser usadas para melhorar os atributos do solo arenoso pobre e, portanto, aumentar a produtividade do solo. De novembro de 2019 a março de 2020, foi realizado um experimento para investigar as taxas de crescimento de buganvílias após bentonita e esterco de galinha em solo arenoso retirado de Lehbab, Dubai. A buganvília foi avaliada quanto à altura da planta (cm), comprimento máximo do ramo primário (cm), número de folhas por planta, número de ramos secundários, peso da parte aérea (g), comprimento da raiz (cm), peso da raiz (g), razão raiz/parte aérea, teores de clorofila e clorofila a* e b*. Neste experimento foi utilizado o delineamento inteiramente casualizado (DIC) com cinco tratamentos (10 repetições por tratamento). De acordo com os resultados, as adições de bentonita e esterco de galinha influenciam consideravelmente as propriedades produtivas do solo arenoso, conforme indicado pelo crescimento de buganvílias. Além disso, a pesquisa sugere que a buganvília pode ser plantada com eficiência com 10% de bentonita e 15% de esterco de galinha aplicado em solo arenoso, resultando, assim, em plantas mais saudáveis em comparação com outras alterações.